Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.
Open Access
- 15 June 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (12), 5124-5128
- https://doi.org/10.1073/pnas.88.12.5124
Abstract
Adenovirus E1A has long been known to activate/repress cellular and viral transcription. The transcriptional activity of nuclear extracts was depleted after chromatography on immobilized E1A protein columns that specifically retained the transcription factor (TF) IID. Stronger direct interactions between E1A and human TFIID than between E1A and yeast TFIID suggest that the unique sequences of the human protein may be involved. We have demonstrated that this interaction occurs directly between bacterially produced E1A and bacterially produced human TFIID in a protein blot assay. We propose that E1A protein may transduce regulatory signals from upstream activators to basal elements of the transcriptional machinery by contacting TFIID.Keywords
This publication has 43 references indexed in Scilit:
- Mechanism of transcriptional activation by Sp1: Evidence for coactivatorsCell, 1990
- A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cellsCell, 1989
- Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complexCell, 1988
- Analysis of the role of the transcription factor ATF in the assembly of a functional preinitiation complexCell, 1988
- A rapid purification method for calf thymus casein kinase IIFEBS Letters, 1988
- An adenovirus E1A protein domain activates transcription in vivo and in vitro in the absence of protein synthesisCell, 1988
- Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase IICell, 1987
- A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoterNature, 1987
- Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box regionCell, 1985
- Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequencesNature, 1983