Regional differences in transport, lipid composition, and fluidity of apical membranes of small intestine of chicken
Open Access
- 1 April 2002
- journal article
- Published by Elsevier in Poultry Science
- Vol. 81 (4), 537-545
- https://doi.org/10.1093/ps/81.4.537
Abstract
Na+-dependent D-glucose transport was studied in brush-border membrane vesicles from duodenum, jejunum, and ileum of 5- to 6-wk-old chickens. Regional differences were found, and both initial rates and accumulation ratio of D-glucose were higher in the proximal part of the small intestine than in the ileum. To establish the mechanism(s) underlying these differences we have studied the density of Na+-dependent D-glucose cotransporter (SGLT1) as well as lipid composition and fluidity. Phlorizin-specific binding and Western blot analysis indicated a decrease in the amount of SGLT1 in the ileum when compared to the duodenum and jejunum. The distal part of the small intestine also showed a decrease in free cholesterol content and saturated-to-unsaturated fatty acid ratio together with an increase in lipid content and phosphatidylcholine-to-sphingomyelin ratio. These results were associated with a decrease in the diphenylhextriene fluorescence polarization found in brush-border membranes of the ileum. We can conclude that the decrease in the apical D-glucose transport found in the ileum is primarily due to a reduction in the amount of SGLT1 present in the brush-border membrane rather than the differences in the lipid composition and fluidity.Keywords
This publication has 17 references indexed in Scilit:
- Hexose transport in the apical and basolateral membranes of enterocytes in chickens adapted to high and low NaCl intakesThe Journal of Physiology, 1999
- Membrane fluidity and transport properties in epitheliaKidney International, 1992
- Ontogenetic Development of Intestinal Nutrient TransportersAnnual Review of Physiology, 1989
- Expression cloning and cDNA sequencing of the Na+/glucose co-transporterNature, 1987
- Sensitivity of Na+-coupled d-glucose uptake, Mg2+-ATPase and sucrase to perturbations of the fluidity of brush-border membrane vesicles induced by n-aliphatic alcoholsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1984
- The simultaneous preparation of basolateral and brush-border membrane vesicles from guinea-pig intestinal epithelium, and the determination of the orientation of the basolateral vesiclesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- Simultaneous isolation of brush border and basolateral membrane from rabbit enterocytes. Presence of brush border hydrolases in the basolateral membrane of rabbit enterocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1980
- Functional interactions of lipids and proteins in rat intestinal microvillus membranesBiochemistry, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Method for assay of intestinal disaccharidasesAnalytical Biochemistry, 1964