Mechanisms of R Factor R931 and Chromosomal Tetracycline Resistance in Pseudomonas aeruginosa
Open Access
- 1 May 1973
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 3 (5), 638-641
- https://doi.org/10.1128/aac.3.5.638
Abstract
The mechanism of tetracycline resistance mediated by R931 (a Pseudomonas aeruginosa R factor not yet successfully transferred to Escherichia coli recipients) was examined. In strain 931 (R931) (minimal inhibitory concentration [MIC] 200 μg/ml) significant tetracycline uptake did not occur until 100 μg of tetracycline per ml was included in uptake studies. The introduction of R931 into strain 280 resulted in a significant decline in 3 H-tetracycline uptake. In both strains 931 (R931) and 280 (R931), a further reduction in tetracycline uptake resulted from pre-incubation with 1 μg of tetracycline per ml. Tetracycline resistance in R − P. aeruginosa strains 1731, 1885, and 494, considered to be of chromosomal origin, was associated with a lack of tetracycline uptake until the MIC of the strain was obtained. No evidence of tetracycline inactivation or ribosomal resistance was detected in R − or R + strains. The MIC for R − strains was generally about 25 μg/ml and that for R + strains was 75 to 200 μg/ml.Keywords
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