COMPARISON OF THE METABOLISM OF AMMONIA AND MOLECULAR NITROGEN IN PHOTOSYNTHESIZING BACTERIA
- 1 May 1952
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 63 (5), 563-573
- https://doi.org/10.1128/jb.63.5.563-573.1952
Abstract
Chromatium sp., Chlorobacterium sp., and Rhodospirilium rubrum, were supplied with N15-enriched N2 for short periods, and a culture of Chromatium also was given N15-enriched NH3. The bacterial cells were recovered and hydrolyzed, and the amino acids and NH3 from the hydrolysates were isolated by chromatographic procedures. Detn. of the distribution of the N15 inducated in every instance that the highest concn. of N15 was in glutamic acid. The NH3 fraction always had the next highest concn. This distribution of N15 in NH3, glutamic acid, and the other amino acids is similar to that found earlier in Azotobacter, Clostridium, and the nodules of leguminous plants when supplied the isotope. The data indicate that NH3 is a key intermediate in biological N-fixation by the photosynthetic bacteria.Keywords
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