Simultaneous Determination of Chloroquine and Desethylchloroquine in Blood, Plasma and Urine by High-Performance Liquid Chromatography
- 1 June 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 6 (8), 1513-1522
- https://doi.org/10.1080/01483918308064868
Abstract
A high-performance liquid chromatographic method is described for the determination of chloroquine and its major metabolite desethylchloroquine in blood, plasma and urine. The procedure employs reversed-phase chromatography, with ultraviolet detection, and chlorpheniramine as an internal standard. One milliliter samples of biologic fluid are extracted in a single step with ether. The method has a sensitivity limit of 5 ng/ml for chloroquine and its metabolite. The applicability of the method is demonstrated by the analysis of blood and plasma samples obtained from rabbits following intravenous administration of chloroquine.This publication has 4 references indexed in Scilit:
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- STUDIES OF METABOLISM OF SOME COMPOUNDS OF 4-AMINO-7-CHLOROQUINOLINE SERIES1966