An evaluation of ways of using equilibrium dialysis to quantify the binding of ligand to macromolecule

Abstract

The effect of systematic error (loss of ligand, complex or macromolecule) on 3 of the experimental designs by which equilibrium dialysis may be used to quantify the interaction of ligand and macromolecule is examined theoretically, and the design that is least sensitive to systematic error is identified. Thirteen methods for fitting the binding isotherm to experimental data are compared by using them to analyze simulated data containing random error, and the most reliable method is identified.