INTERACTION OF NUCLEOSIDES AND RELATED COMPOUNDS WITH NUCLEIC ACIDS AS INDICATED BY THE CHANGE OF HELIX-COIL TRANSITION TEMPERATURE
- 1 April 1962
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 48 (4), 686-698
- https://doi.org/10.1073/pnas.48.4.686
Abstract
A series of compounds has been tested for effectiveness in lowering the melting temperature of poly A and of thymus DNA. The order of increasing activity was found to be adonitol, methyl riboside (both negligible) < cyclohexanol < phenol, pyrimidine, uridine < cytidine, thymidine < purine, adenosine, inosine, deoxyguanosine < caffeine, coumarin, 2,6-dichloro-7-methylpurine. Urea was ineffective with poly A and only slightly effective with DNA. At a concentration of 0.3 [image], purine lowered the Tm of DNA about 9[degree].Keywords
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