FURTHER STUDIES ON DEOXYRIBONUCLEIC ACID-DEPENDENT ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID

Abstract
The incorporation of ATP-C14 into RNA by partially purified enzyme preparations from Lactobacillus arabinosus and Azotobacter vinelandii required the presence of each of the 3 triphosphates of guanosine, uridine, and cytidine and was fully dependent on the presence of native, double-stranded DNA. The 3 samples of DNA investigated, namely L. arabinosus, A. vinelandii, and calf thymus DNA, lost most of their activity after heat denaturation. Heat-denatured L. arabinosus DNA partially inhibited the incorporation brought about by the native compound.