FURTHER STUDIES ON DEOXYRIBONUCLEIC ACID-DEPENDENT ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID
- 1 June 1961
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 47 (6), 749-752
- https://doi.org/10.1073/pnas.47.6.749
Abstract
The incorporation of ATP-C14 into RNA by partially purified enzyme preparations from Lactobacillus arabinosus and Azotobacter vinelandii required the presence of each of the 3 triphosphates of guanosine, uridine, and cytidine and was fully dependent on the presence of native, double-stranded DNA. The 3 samples of DNA investigated, namely L. arabinosus, A. vinelandii, and calf thymus DNA, lost most of their activity after heat denaturation. Heat-denatured L. arabinosus DNA partially inhibited the incorporation brought about by the native compound.Keywords
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