Gas-liquid chromatographic determination of alpha-, beta-, gamma- and delta-BHC levels in human blood, depot fat and various organs with the use of 2,2-dimethylpropane-1,3-diol succinate as the stationary liquid phase

Abstract

The presence of various BHC isomers in the human organism has received relatively little attention, and studies were often restricted to only one BHC isomer. A previously described one-step extraction and clean-up procedure before gas-liquid chromatographic determination of organochlorine pesticide residues in human blood has now been applied to various organs and depot fat. The identification of the BHC isomers was performed by gas-liquid chromatography with several stationary liquid phases. 2,2-Dimethylpropane-1,3-diol succinate was found to be the most satisfactory stationary liquid phase for the present purpose, as it gave distinctly separated peaks and characteristic relative retention times.