Expression ofPseudomonas phosphotriesterase activity in the fall armyworm confers resistance to insecticides
- 1 July 1990
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 46 (7), 729-731
- https://doi.org/10.1007/bf01939948
Abstract
The gene encoding for the phosphotriesterase (opd) fromPseudomonas diminuta has been subcloned into a baculovirus expression system. Functional enzyme is produced when the recombinant baculovirus is used to infect either culturedSpodoptera frugiperda sf9 cells or the larval stage of the fall armyworm. The LD50 for paraoxon toxicity was found to increase 280-fold in the larvae after infection with the recombinant baculovirus and expression of the functional phosphotriesterase.This publication has 11 references indexed in Scilit:
- Purification and Properties of the Phosphotriesterase from Pseudomonas diminutaJournal of Biological Chemistry, 1989
- Structure-activity relationships in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminutaBiochemistry, 1989
- High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectorsVirology, 1989
- Cloning and sequencing of a plasmid-borne gene (opd) encoding a phosphotriesteraseJournal of Bacteriology, 1988
- Mechanism and stereochemical course at phosphorus of the reaction catalyzed by a bacterial phosphotriesteraseBiochemistry, 1988
- Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria.1983
- Stereoselectivity of Organophosphorus InsecticidesPublished by Elsevier ,1982
- An Analysis of Quantal Response Data in Which the Measurement of Response is Subject to ErrorBiometrics, 1965
- An Alternative System for the Classification of Mathematical Models for Quantal Responses to Mixtures of Drugs in Biological AssayBiometrics, 1965
- GRAPHICAL ESTIMATION OF RELATIVE POTENCY FROM QUANTAL RESPONSES1952