Biochemical Correlates of Cardiac Hypertrophy: III. CHANGES IN DNA CONTENT; THE RELATIVE CONTRIBUTIONS OF POLYPLOIDY AND MITOTIC ACTIVITY
- 1 October 1969
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 25 (4), 463-471
- https://doi.org/10.1161/01.res.25.4.463
Abstract
DNA concentration in the left ventricle of 3-month-old rats 3 to 12 days after aortic constriction was similar to the values obtained in sham-operated litter mates (1.38 ± 0.03 µg/mg wet weight). The total DNA content of the heart was thus increased proportionally to its enlargement. In chronic hypertrophy (2 to 4 months after aortic constriction), the DNA content was increased to a significantly less extent than in short-term hypertrophy. The percentage of polyploid nuclei in 12- to 13-week-old rats increased 1 to 3 weeks after aortic constriction from a normal value of 1.8% to 4.2%. In 17- to 18-week-old rats, polyploidy was present in 3.9% of the nuclei 10 days after banding as compared with 1.6% in normal rats. Mitotic figures were localized almost exclusively outside muscle cells. Mitotic indices in hypertrophied hearts were ten times higher than in controls during the first 3 weeks after aortic constriction.Keywords
This publication has 12 references indexed in Scilit:
- Die DNS-synthese im rattenherzen als funktion des lebensalters. Autoradiographische untersuchungen mit3H-thymidinCellular and Molecular Life Sciences, 1968
- Protein Synthetic Activity of Heart Microsomes and Ribosomes during Left Ventricular Hypertrophy in RabbitsCirculation Research, 1967
- Ventricular Nucleic Acid and Protein Levels with Myocardial Growth and HypertrophyCirculation Research, 1966
- The relative amounts of DNA and concentrations of RNA in heart muscle of normal and hypertrophied heartsAmerican Heart Journal, 1964
- Experimental Cardiac Hypertrophy: Concentrations of RNA in the VentriclesScience, 1964
- Deoxyribonucleic Acid Content (Feulgen Photometry) and Dry Weight (Interference Microscopy) of Normal and Hypertrophic Heart Muscle FibresNature, 1964
- Über den Nukleinsäuregehalt des normalen und hypertrophischen KaninchenherzensCellular and Molecular Life Sciences, 1959
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956
- MICROPHOTOMETRY WITH VISIBLE LIGHTPublished by Elsevier ,1956
- The origin of binucleate and large mono nucleate cells in the liver of the ratThe Anatomical Record, 1942