DETERMINATION OF ANTI-DNA ANTIBODIES BY A MODIFIED I-125-LABELED DNA-BINDING TEST - ELIMINATION OF NONSPECIFIC BINDING OF DNA TO NON-IMMUNOGLOBULIN BASIC-PROTEINS BY USING AN ANIONIC DETERGENT
- 1 January 1976
- journal article
- research article
- Vol. 26 (3), 425-430
Abstract
A modified 125I-labeled DNA-binding test was developed. Using an anionic detergent, 0.025% sodium dodecyl sulfate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE [systemic lupus erythematosus] patients.This publication has 16 references indexed in Scilit:
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