Origin and direction of synthesis of bacteriophage fl DNA.
Open Access
- 1 July 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (7), 2341-2345
- https://doi.org/10.1073/pnas.73.7.2341
Abstract
The origin and direction of synthesis in vivo [in infected Escherichia coli] of the viral and complementary strands of f1 DNA were studied by measuring the distribution of radioactivity along the genome after a short pulse of [3H]thymidine. The replication origins of viral and complementary strands are located close to one another, probably both within a restriction fragment (HaeIII-G) which is .apprx. 120 bases long. Replication of viral and complementary strands proceeds in the 5'' .fwdarw. 3'' overall direction. Thus the viral strand is elongated in the counterclockwise and the complementary strand in the clockwise direction on the standard genetic map. A model is proposed in which only 2 mechanisms are invoked to generate all f1 DNA species: the conversion of single-stranded viral DNA into double-stranded molecules and the synthesis of viral single strands from double strands.This publication has 21 references indexed in Scilit:
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