Restriction endonuclease cleavage map of the DNA of JC virus

Abstract
A physical map of the sites cleaved by the following restriction endonucleases was derived for the DNA of JC virus, a human polyomavirus: EcoRI, HpaI and PstI (1 site each); HindII (4 sites); and HindIII (3 sites). By agarose gel electrophoresis of fragmented DNA, the size of full-length DNA of JC virus was estimated to be 5125 .+-. 105 base pairs (98 .+-. 2% of the length of SV-40 DNA).