Gastric Secretion in the Mouse

Abstract

The basal and stimulated gastric secretion of unanesthetized Holtzman substrain of Rockland RAP white Swiss male mice was investigated. Intubation of the stomach with a plastic tube yielded low values of secretion and acidity due to irritation and mucus production in the stomach. Simple ligation of the pylorus demonstrated that the mouse has a constant basal secretion. Histamine stimulated acid production only when employed in very large doses. Best results were obtained when 0.7 mg of histamine dihydrochloride/gm of body weight (70 mg/kg) was injected subcutaneously. Experiments with ligated pylorus of more than 6-hour duration demonstrated lower acidities, and 24-hour experiments showed no free acid, low total acidity and pH near neutrality.