Adsorption and Penetration of Enveloped Herpes Simplex Virus Particles Modified by Tunicamycin or 2-Deoxy-D-glucose

Abstract
3H-labeled, purified herpes simplex virus (HSV) enveloped particles produced in the presence of tunicamycin (TM) or 2-deoxy-D-glucose (DG) adsorbed to African green monkey kidney GMK cells equally as well as standard virus. In the presence of TM or DG, there was a reduced transport of virus DNA to cell nuclei and an increased sensitivity of attached particles to proteinase K. The reduced infectivity of HSV produced in the presence of glycosylation inhibitors is therefore probably due to an impairment in the fusion of virus envelope with plasma membranes.