Effect of fatty acids on the membrane fluidity of cultured chick dorsal root ganglion measured by fluorescence photobleaching recovery
- 1 November 1983
- journal article
- Published by Wiley in Journal of Neurobiology
- Vol. 14 (6), 457-461
- https://doi.org/10.1002/neu.480140605
Abstract
The effect of fatty acids on the membrane fluidity in tissue cultured chick embryo dorsal root ganglion was studied by fluorescence recovery method. Lateral motion of the lipid was measured by observing the fluorescent probe, 5‐(octadecylthiocarbamoylamino) fluorescence, F18. The effective lateral diffusion coefficient of the membrane was around 0.30 × 10−8 cm2/sec in control cells, 0.42 × 10−8 cm2/sec in 2‐decenoic acid treated cells, and 0.35 × 10−8 cm2/sec in valeric acid treated cells. From these results it is concluded that effective mobilities of the membrane complex increased about 40% by the external application of 2‐decenoic acid, while valeric acid increased it only 12%. From the physiological results that 2‐decenoic acid inhibits the Na‐channel, it is suggested that this increase in the membrane fluidity might affect the Na‐channel.Keywords
This publication has 5 references indexed in Scilit:
- The change in membrane fluidity of neuroblastoma × glioma hybrid cells upon cell differentiationFEBS Letters, 1982
- Inhibitory effects of myrmicacin on the sodium channel in the squid giant axon.Proceedings of the Japan Academy, Series B, 1981
- A fluorescence enhancement assay of cell fusionJournal of Cell Science, 1977
- Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts.Proceedings of the National Academy of Sciences, 1976
- Measurement of the translation mobility of concanavalin a in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleachingBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976