Properties of Streptococcus mutans Ingbritt growing on limiting sucrose in a chemostat: repression of the phosphoenolpyruvate phosphotransferase transport system
- 1 May 1982
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 36 (2), 576-581
- https://doi.org/10.1128/iai.36.2.576-581.1982
Abstract
Growth of Streptococcus mutans Ingbritt on limiting sucrose in a chemostat at dilution rates of 0.05 to 0.4 h-1 (mean generation time, 14 to 1.7 h) resulted in a heterofermentative pattern of metabolic end products. During fast growth, lactic acid was the major end product, whereas at slower growth rates, acetic and formic acids, as well as ethanol, increased to be major end products. The patterns obtained were similar to those seen with the same organism growing on glucose. The glycolytic rate by washed cells was maximum at the lowest dilution rates and decreased as the cells were made to grow faster. Transport of sucrose, glucose, and fructose via the phosphoenolpyruvate phosphotransferase system (PTS) was repressed during growth on sucrose after growth on glucose. Uptake rates suggested that sucrose was transported in the PTS as the intact disaccharide. Comparison of the rate of sugar uptake in the chemostat with the rate of PTS activity in the cells at each growth rate indicated that the PTS was capable of supporting growth only at a dilution rate of 0.05 h-1. Growth on sucrose at faster growth rates required the activity of a second transport system, supporting our earlier observations with glucose that S. mutans contains at least two sugar transport systems.This publication has 17 references indexed in Scilit:
- Evidence for the Involvement of Proton Motive Force in the Transport of Glucose by a Mutant of Streptococcus mutans Strain DR0001 Defective in Glucose-Phosphoenolpyruvate Phosphotransferase ActivityInfection and Immunity, 1982
- Association of Streptococcus mutants with human dental decay.1975
- Fermentation products and bacterial yields in glucose-limited and nitrogen-limited cultures of streptococciArchives of Oral Biology, 1974
- Growth of Bacteroides fragilis in continuous culture and in batch cultures at controlled pH.1974
- Growth of Streptococcus mutans in a chemostatArchives of Oral Biology, 1974
- Changes in Streptococcus mutans and lactobacilli in plaque in relation to the initiation of dental caries in negro childrenArchives of Oral Biology, 1973
- Inducible phosphoenolpyruvate-dependent hexose phosphotransferase activities in Escherichia coliBiochemical Journal, 1972
- Sucrose, the arch criminal of dental caries.1967
- The effects of some carbohydrates on experimental dental caries in the ratArchives of Oral Biology, 1963
- The Effect of Different Levels of Carbohydrate Intake on Caries Activity in 436 Individuals Observed for Five YearsActa Odontologica Scandinavica, 1953