Regenerable Bacterial Membrane Electrode for L-Aspartate

Abstract

The use of living bacteria held at the surface of an NH₃ gas sensing membrane electrode permits the construction of a potentiometric sensor for L-aspartate. It is shown that the bacterial electrode, when stored in nutrient media, can have an appreciably longer lifetime than the corresponding electrode based on isolated L-aspartase enzyme. The response range and dynamic properties of the bacterial electrode are comparable to those of the enzyme electrode.