Poly(A)‐Containing RNA in Tetrahymena pyriformis
- 1 December 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 71 (1), 117-124
- https://doi.org/10.1111/j.1432-1033.1976.tb11096.x
Abstract
Poly(A)-containing RNA with sedimentation coefficients in the range 10-45 S and 10-35 S (Mr [molecular ratio] 0.2-3.3 .times. 106 and .apprx. 0.2-2.2 .times. 106) are present in the nucleus and cytoplasm of T. pyriformis, respectively. The poly(A) segments present in both nuclear and cytoplasmic poly(A)-containing RNA have a chain length of 80-150 nucleotides. Significant amounts of high-molecular-weight poly(A)-containing RNA (35 S, Mr 2.2 .times. 106) are found in the post-polysomal fraction of the cytoplasm (messenger ribonucleoprotein particles) and also in large polysomes. In order to exclude the possibility that these high-molecular-weight species are products of non-specific aggregation, RNA preparations were submitted to severe denaturing conditions before analysis and all electrophoreses were carried out in the presence of 95% vol/vol formamide.This publication has 42 references indexed in Scilit:
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