MANNITOL ESTIMATION IN BIOLOGICAL-FLUIDS BY GAS-LIQUID-CHROMATOGRAPHY OF TRIMETHYLSILYL DERIVATIVES

Abstract

A procedure is described for estimating mannitol concentrations in biological fluids. Samples are mixed with internal standard solution (.alpha.-methylglucose), deproteinized if necessary, desalted and dried. Specimens are then derivatized by adding pyridine/bis(trimethylsilyl)acetamide/trimethylchlorosilane and heating at 60.degree. C for 30 min. Samples are chromatographed on a 275-cm column of 10% OV-17, operated at 190.degree. C and quantitated by peak-height measurement. The technique is linear, accurate, precise, sensitive and free from interference. It was used to measure mannitol in plasma, urine and bile.