A method for exposing the rat renal medulla in vivo: micropuncture of the collecting duct
- 31 August 1965
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Legacy Content
- Vol. 209 (3), 663-668
- https://doi.org/10.1152/ajplegacy.1965.209.3.663
Abstract
A new method involving partial nephrectomy is described by which the distal portion of the rat renal medulla is made accessible to an investigation by the micropuncture technique. Urinary flow was greater and osmolality less in the partially nephrectomized left kidney than the unoperated right kidney but the urine remained hypertonic. Micropuncture of the collecting duct revealed a rising osmotic gradient toward the papillary tip. Electrical potential differences across the collecting ducts were measured by microelectrode techniques. The lumen was electrically negative with respect to the interstitial fluid (mean - 11 mv). It is clear that medullary hypertonicity persists in the partially nephrectomized left kidney and the collecting ducts retain their ability to reabsorb water and maintain a transtubular potential difference. In these aspects of tubular activity, the exposed rat renal medulla appears to be similar to that of the hamster, but has the advantage of a greater exposure of its length to investigation by micropuncture.Keywords
This publication has 14 references indexed in Scilit:
- Freezing-Point Depression: New Method for Measuring Ultramicro Quantities of FluidsScience, 1963
- Micropuncture study of composition of loop of Henle fluid in desert rodentsAmerican Journal of Physiology-Legacy Content, 1963
- Effect of unreabsorbed anions on proximal and distal transtubular potentials in ratsAmerican Journal of Physiology-Legacy Content, 1962
- EVIDENCE FOR ACTIVE CHLORIDE REABSORPTION IN THE DISTAL RENAL TUBULE OF THE RAT*JCI Insight, 1962
- Short-circuit current measurements in proximal tubule of Necturus kidneyAmerican Journal of Physiology-Legacy Content, 1961
- Micropuncture study of net transtubular movement of water and urea in nondiuretic mammalian kidneyAmerican Journal of Physiology-Legacy Content, 1961
- Micropuncture study of the mammalian urinary concentrating mechanism: evidence for the countercurrent hypothesisAmerican Journal of Physiology-Legacy Content, 1959
- The effect of internal and external potassium concentration on the membrane potential of frog muscleThe Journal of Physiology, 1956
- METHODS FOR THE COLLECTION OF FLUID FROM SINGLE GLOMERULI AND TUBULES OF THE MAMMALIAN KIDNEYAmerican Journal of Physiology-Legacy Content, 1941
- METHODS OF COLLECTING FLUID FROM KNOWN REGIONS OF THE RENAL TUBULES OF AMPHIBIA AND OF PERFUSING THE LUMEN OF A SINGLE TUBULEAmerican Journal of Physiology-Legacy Content, 1936