A Rapid High-Performance Liquid Chromatographic Method for Quantitation of 5-Fluorouracil in Plasma after Continuous Intravenous Infusion

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Abstract
A fast, simple, precise, high-performance liquid chromatographic method for the assay of 5-fluorouracil in plasma from humans receiving continuous intravenous infusion is described. After a single extraction, underivatised 5-fluorouracil is assayed by high-performance liquid chromatography using a variable-wavelength ultraviolet detector. After extraction of 1 ml plasma, 0·3 μmol/l can be assayed. The assay is reproducible and linear up to at least 40 μmol/l. A single determination takes 3 hours and a batch of 40 specimens can be extracted and assayed in 4 working hours if automated equipment is used, enabling injections and calculations to be done overnight.