Synteny between the Pro+ marker and human glutamate oxaloacetate transaminase
- 1 October 1975
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 1 (4), 345-354
- https://doi.org/10.1007/bf01538666
Abstract
Chinese hamster ovary cells with a specific auxotrophy for proline were fused with human cells from a variety of sources and the resulting hybrids analyzed for human genetic markers. Of 63 hybrid clones examined, 27 possessed both proline and cytoplasmic glutamate oxaloacetate transaminase markers; 36 had neither; and no clones were found possessing one and not the other. These results constitute evidence that the proline and glutamate oxalocetate transaminase markers are syntenic. Evidence for absence of synteny between these and a variety of other human genes is presented. Biochemical tracer experiments established that the proline biosynthetic pathway through glutamate has been restored in the Pro+ hybrids.Keywords
This publication has 15 references indexed in Scilit:
- Proline biosynthesis: Multiple defects in Chinese hamster ovary cellsBiochemical and Biophysical Research Communications, 1973
- SURFACE ANTIGENS OF MAMMALIAN CELLS AS GENETIC MARKERS. IIThe Journal of Experimental Medicine, 1973
- Assignment of the genes for human peptidase A to chromosome 18 and cytoplasmic glutamic oxaloacetate transaminase to chromosome 10 using somatic-cell hybridsCytogenetic and Genome Research, 1973
- Genetics of somatic mammalian cells. XV. Evidence for linkage between human genes for lactic dehydrogenase B and serine hydroxymethylaseJournal of Cellular Physiology, 1972
- Genetics of somatic mammalian cells. XIV. Genetic analysisin vitro of auxotrophic mutantsJournal of Cellular Physiology, 1972
- Genetics of Somatic Mammalian Cells: Linkage Studies with Human–Chinese Hamster Cell HybridsNature, 1970
- Complementation Analysis on Virus-Fused Chinese Hamster Cells with Nutritional MarkersScience, 1969
- CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUMProceedings of the National Academy of Sciences, 1965
- An improved nutrient solution for diploid Chinese hamster and human cell linesExperimental Cell Research, 1963
- [9] Quantitative colonial growth of isolated mammalian cellsMethods in Enzymology, 1962