Factors affecting recovery of latent herpes simplex virus from human trigeminal ganglia
- 1 January 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 28 (1), 123-129
- https://doi.org/10.1139/m82-013
Abstract
The rate of recovery of herpes simplex virus (HSV) from human trigeminal ganglia explant monolayers is affected by two factors: (1) time elapsed from the death of an individual to the establishment of in vitro culture of ganglia and (2) surface area onto which ganglia are explanted. Spontaneous reactivation of HSV from human trigeminal ganglia can be maximized when ganglia are obtained within 12 h of death and explanted onto a surface area of 250 cm2. Viruses isolated by explantation of human trigeminal ganglia were found to be uniformly HSV type 1 by restriction enzyme analysis.This publication has 5 references indexed in Scilit:
- Detection by complementation of defective or uninducible (herpes simplex type 1) virus genomes latent in human ganglia.Proceedings of the National Academy of Sciences, 1979
- A RAPID TECHNIQUE FOR DISTINGUISHING HERPES-SIMPLEX VIRUS TYPE 1 FROM TYPE 2 BY RESTRICTION-ENZYME TECHNOLOGYThe Lancet, 1979
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- Isolation of Latent Herpes Simplex Virus from the Superior Cervical and Vagus Ganglions of Human BeingsNew England Journal of Medicine, 1978
- ISOLATION OF HERPES SIMPLEX VIRUS FROM HUMAN TRIGEMINAL GANGLIA, INCLUDING GANGLIA FROM ONE PATIENT WITH MULTIPLE SCLEROSISThe Lancet, 1977