Reductive dehydroxylation of 4‐hydroxybenzoyl‐CoA to benzoyl‐CoA in a denitrifying, phenol‐degrading Pseudomonas species
- 17 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2), 237-240
- https://doi.org/10.1016/0014-5793(89)81461-9
Abstract
The initial reactions in anaerobic degradation of phenol to CO2 have been studied in vitro with a denitrifying Pseudomonas strain grown with phenol and nitrate in the absence of molecular oxygen. Phenol has been proposed to be carboxylated to 4-hydroxybenzoate [(1987) Arch. Microbiol. 148, 213–217]. 4-Hydroxybenzoate was activated to 4-hydroxybenzoyl-CoA by a coenzyme A ligase. Cell extracts also catalyzed the reductive dehydroxylation of 4-hydroxybenzoyl-CoA to benzoyl-CoA with reduced benzyl viologen as electron donor. This enzyme, benzoyl-CoA:(acceptor) 4-oxidoreductase (hydroxylating) (EC 1.3.99.-), has not been reported before. The data suggest that phenol and 4-hydroxybenzoate are anaerobically metabolized by this strain via benzoyl-CoA.Keywords
This publication has 19 references indexed in Scilit:
- Degradation of hydroquinone, gentisate, and benzoate by a fermenting bacterium in pure or defined mixed cultureArchiv für Mikrobiologie, 1989
- Degradation of phenol via carboxylation to benzoate by a defined, obligate syntrophic consortium of anaerobic bacteriaApplied Microbiology and Biotechnology, 1989
- Anaerobic Degradation of Aromatic CompoundsAnnual Review of Microbiology, 1988
- Studies on the anaerobic degradation of benzoic acid and 2-aminobenzoic acid by a denitrifying Pseudomonas strainArchiv für Mikrobiologie, 1987
- Fermentative degradation of monohydroxybenzoates by defined syntrophic coculturesArchiv für Mikrobiologie, 1986
- Enterobacter cloacae DG-6: a strain that transforms methoxylated aromatics under aerobic and anaerobic conditionsCurrent Microbiology, 1985
- Selenium-Dependent EnzymesAnnual Review of Biochemistry, 1980
- Biochemistry of the bacterial catabolism of aromatic compounds in anaerobic environmentsNature, 1977
- Study of the intestinal tyrosine metabolism using stable isotopes and gas chromatography-mass spectrometryJournal of Chromatography A, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976