Simplified Method for Determination of Total Adrenal Cholesterol.

Abstract

The method of Zlatkis, Zak and Boyle (J. Lab. and Clin. Med., 1953, 41: 486) for the direct determination of serum cholesterol was adapted to determination of total adrenal cholesterol. The values thus obtained do not differ significantly from those obtained by the method of Sperry and Webb in normal, hypophysectomized and cold stressed rats. The time required to analyze 10 samples and 5 standards by the Zlatkis, Zak and Boyle procedure requires less than 1 hour compared to the 2 days usually necessary when methods based on the Liebermann-Burchard reaction are utilized.