Initiation Factor 2-Dependent Ribosomal Binding of N -Formylmethionyl-Transfer RNA Without Added Guanosine Triphosphate
- 1 October 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (10), 2770-2773
- https://doi.org/10.1073/pnas.69.10.2770
Abstract
Evidence is presented that suggests that GTP (or 5'-guanylylmethylene diphosphonate) is not essential for either formation of an AUG-directed, initiation factor IF-2-dependent initiation complex on the 30S subunit or for positioning of fMet-tRNA on the peptidyl site. However, recycling of limiting amounts of IF-2 requires both GTP and the 50S subunit. The formation of the complex IF-2-30S as an intermediate in polypeptidechain initiation is suggested.Keywords
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