Glucocorticoid Action on Hybrid Clones Derived from Cultured Myeloma and Lymphoma Cell Lines
- 1 November 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (11), 3124-3127
- https://doi.org/10.1073/pnas.69.11.3124
Abstract
Cells of a cloned myeloma line from a Balb/c mouse contain specific cytoplasmic glucocorticoid receptors and are killed by dexamethasone. Cells of a lymphoma line (from mouse strain C57BL) also contain specific glucocorticoid receptors but are resistant to the steroid. Cells of two hybrid clones with widely differing chromosome numbers, derived by fusion between the resistant lymphoma and the sensitive myeloma, contain specific glucocorticoid receptors with similar binding properties as the parental receptors and are killed by dexamethasone. Since the lethal effect of the steroid is expressed in the hybrid cells, failure of the parent lymphoma line to be affected by dexamethasone is probably not due to an inhibitor of the lethal reaction.Keywords
This publication has 24 references indexed in Scilit:
- Glucocorticoid receptors: Relations between steroid binding and biological effectsJournal of Molecular Biology, 1972
- THYMUS-MARROW IMMUNOCOMPETENCEThe Journal of Experimental Medicine, 1971
- Glucocorticoid Receptors in Lymphoma Cells in Culture: Relationship to Glucocorticoid Killing ActivityScience, 1971
- Hybrid Cell Line from a Cloned Immunoglobulin-Producing Mouse Myeloma and a Nonproducing Mouse LymphomaScience, 1971
- Relation of steroid structure to enzyme induction in hepatoma tissue culture cellsJournal of Molecular Biology, 1970
- Differentiated functions expressed by cultured mouse lymphoma cells: I. Specificity and kinetics of cell responses to corticosteroidsExperimental Cell Research, 1970
- Mouse myelomas and lymphomas in cultureExperimental Cell Research, 1970
- Specificity and thermodynamic properties of the corticosteroid binding to a receptor of rat thymocytes in vitroBiochimica et Biophysica Acta (BBA) - General Subjects, 1968
- Biochemical and Biophysical Research Communications, 1966
- Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line.Proceedings of the National Academy of Sciences, 1966