Characterization of Bacterial Growth by Means of Flow Microfluorometry
- 16 December 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 198 (4322), 1175-1176
- https://doi.org/10.1126/science.412254
Abstract
By means of flow microfluorometry, the protein and nucleic acid contents of individual bacterial cells may be measured at the rate of several thousand cells per second. Accumulation of such information over a few minutes yields the composition distribution of the microbial population. These distributions have been determined at different times during batch growth of Bactillus subtilis, and the results indicate that the variance of cell composition decreases as the population passes through the exponential into the stationary phase. The relative abundance of endospores and vegetative cells as well as the protein distributions of these subpopulations may be readily determined from flow microfluorometry data. Experimental access to such details of microbial population dynamics should foster improved understanding of cell growth, spore germination, and spore formation kinetics.This publication has 8 references indexed in Scilit:
- Lasers in Biomedicine: Analyzing and Sorting CellsScience, 1975
- Chapter 12 Methods and Applications of Flow Systems for Analysis and Sorting of Mammalian CellsMethods in Cell Biology, 1975
- A New Multiparameter Separator for Microscopic Particles and Biological CellsReview of Scientific Instruments, 1973
- Continuous production of extracellular protease by Bacillus subtilis in a two‐stage fermentorBiotechnology & Bioengineering, 1972
- THE USE OF AN ETHIDIUM ANALOGUE IN THE DYE-BUOYANT DENSITY PROCEDURE FOR THE ISOLATION OF CLOSED CIRCULAR DNA: THE VARIATION OF THE SUPERHELIX DENSITY OF MITOCHONDRIAL DNAProceedings of the National Academy of Sciences, 1969
- Kinetics of Growth of Individual Cells ofEscherichia coliandAzotobacter agilisJournal of Bacteriology, 1967
- Spectrophotometer: New Instrument for Ultrarapid Cell AnalysisScience, 1965
- Dependency on Medium and Temperature of Cell Size and Chemical Composition during Balanced Growth of Salmonella typhimuriumJournal of General Microbiology, 1958