INCREASED ADRENOCORTICOTROPHIC HORMONE IN THE SERA OF ACUTELY SCORBUTIC GUINEA-PIGS
- 1 July 1957
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 15 (3), 284-296
- https://doi.org/10.1677/joe.0.0150284
Abstract
SUMMARY: 1. In the Sayers test for adrenocorticotrophic hormone (ACTH), serum obtained from normal guinea-pigs was not active. Serum from guinea-pigs with severe scurvy caused ascorbic acid (AA) depletion and behaved as though it contained ACTH. The response increased with increasing dosage, and a valid assay was obtained with it and with authentic ACTH dissolved in serum from normal animals. Both normal serum and that from scorbutic animals sometimes possessed the property of increasing the slope of the response curve above that obtained for ACTH in saline. 2. 100 ml. of a sample of pooled sera from guinea-pigs with severe scurvy was estimated to possess the activity of 41 mU ACTH, but the fiducial limits were wide. 3. When guinea-pigs, given a small daily supplement of AA, were pair-fed with animals developing scurvy, no activity was detected in their sera. Activity was reduced and perhaps abolished in the sera of animals with acute scurvy by treatment in the terminal stages with AA, cortisol, cortisone or deoxycorticosterone acetate (DCA), but not with progesterone, oestradiol or testosterone. 4. During the development of scurvy the urinary 17-ketosteroids (KS) and 17-ketogenic steroids (KG) rose steadily, but in animals pair-fed with them and receiving a small supplement of AA each day, the increase in KS was less and KG showed little change.Keywords
This publication has 21 references indexed in Scilit:
- PRELIMINARY STUDIES ON BLOOD ACTH IN MAN*Journal of Clinical Endocrinology & Metabolism, 1953
- ASSESSMENT OF ADRENOCORTICAL ACTIVITY BY ASSAY OF 17-KETOGENIC STEROIDS IN URINEThe Lancet, 1953
- ACTION OF ASCORBIC ACID ON THE RESPONSE OF THE PITUITARY-ADRENAL AXIS IN THE GUINEA PIGEndocrinology, 1952
- EXCRETION OF FORMALDEHYDEGENIC (FG) SUBSTANCES BY NORMAL AND SCORBUTIC GUINEA PIGS*Endocrinology, 1952
- Prolongation of Effects of Adrenal Cortical Secretion by Ascorbic Acid: Proposed Mechanism of Action.Experimental Biology and Medicine, 1952
- A Technic for Determination of Adrenocorticotrophin in Blood.Experimental Biology and Medicine, 1952
- INCREASE IN THE FORMATION AND SECRETION OF ACTH FOLLOWING ADRENALECTOMY1Endocrinology, 1951
- ADRENOTROPHIC ACTIVITY OF HUMAN BLOOD1Endocrinology, 1949
- REGULATION OF PITUITARY ADRENOCORTICOTROPHIC ACTIVITY DURING THE RESPONSE OF THE RAT TO ACUTE STRESS1Endocrinology, 1947
- COMPENSATORY ATROPHY OF THE ADRENALSJAMA, 1940