NOTES: Two-Dimensional Thin-Layer Chromatography for Amino Acid Analysis of Bacterial Cell Walls
- 1 January 1979
- journal article
- research article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 29 (1), 56-58
- https://doi.org/10.1099/00207713-29-1-56
Abstract
Good separation of bacterial cell wall amino acids, including the isomers of diaminopimelic acid, was obtained by 2-dimensional TLC using commercial cellulose-coated Al sheets with isopropanol-acetic acid-water (75:10:15, vol/vol) and methanol-pyridine-10 N hydrochloric acid-water (64:8:2:14, vol/vol) as the 1st and 2nd solvents, respectively. [This study relates to bacterial chemotaxonomy.].This publication has 3 references indexed in Scilit:
- The Cell Wall Composition and Distribution of Free Mycolic Acids in Named Strains of Coryneform Bacteria and in Isolates from Various Natural SourcesJournal of Applied Bacteriology, 1977
- The Behavior of the Isomers of α,ε-Diaminopimelic Acid on Paper ChromatogramsJournal of the American Chemical Society, 1955
- Filter-paper partition chromatography of sugarsBiochemical Journal, 1947