Control of normal differentiation of myeloid leukemic cells. XII. Inducibility for some stages of differentiation by dimethylsulfoxide and its disassociation from inducibility by MGI

Abstract

There are clones of myeloid leukemic cells that can be induced to differentiate by the normal differentiation-inducing protein MGI to form Fc and C3 rosettes, mature macrophages and granulocytes. One of these clones (MGI⁺DMSO⁺) was also inducible by dimethylsulfoxide (DMSO) for C3 but not Fc rosettes, and for mature macrophages but not for mature granulocytes. Other clones (MGI⁺DMSO⁻) were inducible by MGI but not DMSO and a third type of clone (MGI⁻DMSO⁻) was not inducible by either compound. Clones that differed in their inducibility by DMSO showed a similar inhibition of cell multiplication by DMSO. The results indicate, that some stages of differentiation can be induced by DMSO in an appropriate clone of myeloid leukemic cells and that there are different cellular sites for induction by DMSO and MGI.