Studies on the biosynthesis of surfactin, a lipopeptide antibiotic from Bacillus subtilis ATCC 21332
- 11 April 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 231 (1), 107-110
- https://doi.org/10.1016/0014-5793(88)80712-9
Abstract
The biosynthesis of the lipopeptide antibiotic surfactin was studied in whole cells of Bacillus subtilis ATCC 21332 which incorporate 14C-labeled precursor amino acids directly into the product. [14C]Acetate appeared in the fatty acid portion of surfactin and was also partially converted into leucine. An enzyme was isolated and partially purified from a cell-free extract of the bacillus which catalyzes ATP-Pi-exchange reactions which are mediated by the amino acid components of surfactin. This activation pattern is consistent with a peptide synthesizing multienzyme which activates its substrate amino acids simultaneously as reactive aminoacyl phosphatesKeywords
This publication has 18 references indexed in Scilit:
- Lipopetides, an attractive class of microbial surfactantsPublished by Springer Nature ,2007
- Acylpeptides, the inhibitors of cyclic adenosine 3',5'-monophosphate phosphodiesterase. II. Amino acid sequence and location of lactone linkage.The Journal of Antibiotics, 1983
- Acylpeptides, the inhibitors of cyclic adenosine 3',5'-monophosphate phosphodiesterase. I. Purification, physicochemical properties and structures of fatty acid residues.The Journal of Antibiotics, 1983
- Nucleic Acid Independent Synthesis of PeptidesPublished by Springer Nature ,1981
- Peptide Bond Formation in Non-ribosomal SystemsPublished by Springer Nature ,1978
- Glutathione and Related γ-Glutamyl Compounds: Biosynthesis and UtilizationAnnual Review of Biochemistry, 1976
- Nonribosomal polypeptide synthesis on polyenzyme templatesAccounts of Chemical Research, 1973
- Surfactin, a crystalline peptidelipid surfactant produced by Bacillussubtilis: Isolation, characterization and its inhibition of fibrin clot formationBiochemical and Biophysical Research Communications, 1968
- [95] Amino acid-activating enzymes: Methods of assayMethods in Enzymology, 1962
- Bacillomycin: An Antibiotic from Bacillus subtilis Active against Pathogenic FungiExperimental Biology and Medicine, 1948