Human apolipoprotein B: partial amino acid sequence

Abstract

A successful approach has been developed for the sequencing of apolipoprotein B based upon the procedure of Cleveland et al. [(1977) J. Biol. Chem. 252, 1102–1106] involving limited proteolysis in the presence of sodium dodecyl sulfate. Staphylococcus aureus protease was employed to produce large peptides which were isolated in relatively pure form by preparative gel electrophoresis. Two peptides were partially sequenced using spinning‐cup microsequencing techniques. The sequences are: Peptide R2‐5, ‐Ala ‐ Leu ‐ Val ‐ Gly ‐ Ile ‐ Asn ‐ Gly ‐ Glu ‐ Ala ‐ Asn ‐ Leu ‐ Asp ‐ Phe ‐ Leu ‐ Asn ‐ Ile ‐ Pro ‐ Leu ‐ Arg‐ Ile ‐ Pro‐ Pro‐Met‐Arg‐(Arg)‐; and Peptide R3‐1, ‐Leu‐Val‐Ala‐Lys‐Pro‐Ser‐Val‐Ser‐Val‐Glu‐Phe‐Val‐Thr ‐Asn‐Met‐Gly‐Ile‐Ile‐Ile‐Pro‐Lys‐Phe‐Ala‐Arg‐. Several stretches of residues suitable for the construction of oligonucleotide probes have been identified.