Negative silver staining in A-T and satellite DNA-rich regions of human chromosomes
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 57 (2), 165-169
- https://doi.org/10.1007/bf00292914
Abstract
Human metaphase chromosomes were stained with silver following a pretreatment with a heated alkaline solution. The most conspicuous feature of the stained metaphases was the omission of silver staining in the secondary constrictions of chromosomes 1, 9 and 16, and on the distal Yq. Our evidence indicates that the negative silver binding is due to the preferential removal or alteration of non-histone proteins associated with these regions. The cytochemical significance of these findings is discussed.Keywords
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