Expression and Self-Assembly of Norwalk Virus Capsid Protein from Venezuelan Equine Encephalitis Virus Replicons
- 15 March 2002
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (6), 3023-3030
- https://doi.org/10.1128/jvi.76.6.3023-3030.2002
Abstract
The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells.Keywords
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