In the previous communication (1) it has been reported that alkaligenic acid is a special kind of nucleic acid isolated from a certain species of Alcaligenes faecalis and contains considerable amounts of sugar-polypeptide complex in a bound form, the latter of which, upon hydrolysis, separates into 9 to 10 amino acids together with glucosamine. The present study deals with the quantitative estimation of its component amino acids and glucosamine according to the method by Moore and Stein (2) with slight modification by the writer. At first mention has been made of the modified points, then followed by the description of the analytical data obtained for alkaligenic acid by this technique.