Investigation of Rose Bengal Conjugation.

Abstract
This study reports that metabolites of Rose Bengal could not be demonstrated in human bile, serum or urine or in extracts from rat liver by a variety of systems involving paper and column chromatography and electrophoresis. Conjugation with cystine-S35 or glucuronic acid was not demonstrable. It was concluded that Rose Bengal is not conjugated by the liver in the same form reported for bilirubin (glucuronides) or bromsulfalein (mercaptides).