Intracellular Location of Carbonate Dehydratase (Carbonic Anhydrase) in Leaf Tissue
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 63 (1), 216-217
- https://doi.org/10.1104/pp.63.1.216
Abstract
Two proteins which have carbonate dehydratase (carbonic anhydrase, EC 4.2.1.1) activity were in the chloroplasts and in the cytosol of leaves of Brassica chinensis, Spinacia oleracea, and in variegated leaves of Tradescantia albiflora and Hedera canariensis. The chloroplastic enzyme is smaller than the one in the cytosol, as it runs farther on gradient polyacrylamide gels. It was separated from the other by isolation of chloroplasts of Brassica and Spinacia on sucrose density gradients; approximately half of the total activity was in the chloroplasts.This publication has 9 references indexed in Scilit:
- Isolation of Intact Chloroplasts and Other Cell Organelles from Spinach Leaf ProtoplastsPlant Physiology, 1976
- [Purification and characterization of chloroplast carbonate dehydratase from leaves of Lactuca sativa (author's transl)].1975
- Carbonic Anhydrase of SpinachPlant Physiology, 1975
- Accumulation of bicarbonate in intact chloroplasts following a pH gradientBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
- Plant Carbonic AnhydrasesPlant Physiology, 1972
- Intracellular distribution of carbonic anhydrase in spinach leavesBiochimica et Biophysica Acta (BBA) - Enzymology, 1972
- Carbonic anhydrase: A new method of detection on polyacrylamide gels using low-temperature fluorescenceAnalytical Biochemistry, 1971
- Evidence for the synthesis in vivo of proteins of the calvin cycle and of the photosynthetic electron-transfer pathway on chloroplast ribosomesBiochemical and Biophysical Research Communications, 1967
- Carbonic Anhydrases from Human ErythrocytesJournal of Biological Chemistry, 1964