IN VITRO PRODUCTION OF TESTOSTERONE AND ANDROST-4-ENE-3,17-DIONE IN A HUMAN OVARIAN HOMOGENATE

Abstract

Testosterone and androst-4-ene-3,17-dione have been isolated and identified by isotope dilution techniques from an incubation of 7-3H-progesterone and 14C-17[alpha]-hydroxyprogesterone with a normal human ovarian homogenate. This offers substantial proof that the production of this highly active androgen is possible under normal physiological circumstances.