A human plasma cell line. Induction and characterization
- 15 May 1982
- Vol. 49 (10), 2091-2096
- https://doi.org/10.1002/1097-0142(19820515)49:10<2091::aid-cncr2820491021>3.0.co;2-9
Abstract
A stable line of IgG K producing human plasma cells was established from a myelomatous human bone marrow using conditioned media from a rapidly metabolizing lymphoblast line, RPMI 4098. Growth in RPMI 1640 (15% fetal calf serum) at 6% CO2 promoted a 62-hour doubling time with a preferred cell concentration of 1 × 106/mL. Surface marker studies showed: no receptors for sheep erythrocytes, no surface immunoglobulins, variable number of cells bearing complement receptors and 83% bearing Fe receptors. Although transmission electron micrographs demonstrated a poorly developed endoplasmic reticulum, radioimmunoassay showed 23 ng IgG and 28.7 ng Kappa were produced by 1 × 106 cells in 72 hours. Further, the cells are lipase, esterase and Epstein-Barr nuclear antigen negative. ASG banding showed a total chromosome number that varied from 46–49. Since the number of human plasma cell lines is limited, it is felt that this line will augment the immunobiological study of human myeloma.This publication has 8 references indexed in Scilit:
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