An Improved method for the purification of tRNA by chromatography on dlhydroxyboryl substituted cellulose
Open Access
- 1 June 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (6), 853-864
- https://doi.org/10.1093/nar/2.6.853
Abstract
An Improved method for the rapid separation of aminoacyl-tRNA from tRNA by chromatography on dihydroxyboryl-substituted cellulose has been developed. The method relies on the selective binding of unacylated tRNA to the cellulose support containing dlhydroxyboryl groups. This binding is the result of complex formation between the cis-diol group of the 3′-terminal ribose in tRNA and the dihydroxyboryl groups immobilized on the resin. Aminoacyl-tRNA cannot undergo borate complex formation and is not retained on the resin. The separation is carried out at near neutral pH values ensuring stability of the aminoacyl ester linkage. The aminoacyl-tRNAs are obtained in very high purity. Aminoacyl-tRNA species containing the modified nucleoside Q are also retained on dihydroxyboryl cellulose. Conditions for Isolating all Q base containing tRNA species from unfractionated tRNA are described.Keywords
This publication has 16 references indexed in Scilit:
- Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase methodBiochemical and Biophysical Research Communications, 1974
- 3-(3-Amino-3-carboxypropyl)uridine. Structure of the nucleoside in Escherichia coli transfer ribonucleic acid that reacts with phenoxyacetoxysuccinimideBiochemistry, 1974
- Purification of chicken liver seryl transfer ribonucleic acid by complex formation with elongation factor EF-Tu:GTP. General micromethod of aminoacyl transfer ribonucleic acid purificationBiochemistry, 1973
- Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locusJournal of Molecular Biology, 1973
- Dihydroxyboryl-substituted methacrylic polymer for the column chromatographic separation of mononucleotides, oligonucleotides, and transfer ribonucleic acidBiochemistry, 1973
- Nucleotide sequence of tRNASer3 from Escherichia coliFEBS Letters, 1973
- Relation between aminoacyl-tRNA stability and the fixed amino acidBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Interactions of nucleotides, polynucleotides, and nucleic acids with dihydroxyboryl-substituted cellulosesBiochemistry, 1972
- Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acidBiochemistry, 1972
- Synthesis of cellulose derivatives containing the dihydroxyboryl group and a study of their capacity to form specific complexes with sugars and nucleic acid componentsBiochemistry, 1970