A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis
- 1 March 1983
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 302 (5905), 223-228
- https://doi.org/10.1038/302223a0
Abstract
The function of the X. laevis ribosomal spacer was studied in vivo and by microinjection of in vitro mutants into Xenopus oocytes. The spacer directs specific RNA transcripts which most probably terminate upstream of the ribosomal genes and it is able to modulate transcription of these genes. The data lead to a model in which the ribosomal spacer is a loading site for RNA polymerase I and spacer transcription is the driving force by which polymerase is delivered to the ribosomal gene promoter.This publication has 26 references indexed in Scilit:
- Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoterCell, 1982
- Expression of a β-globin gene is enhanced by remote SV40 DNA sequencesCell, 1981
- Loss of rDNA methylation accompanies the onset of ribosomal gene activity in early development of X. laevisCell, 1981
- Visualization of rDNA spacer transcription in Xenopus oocytes treated with fluorouridineExperimental Cell Research, 1978
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Restriction analysis of the nontranscribed spacers of Xenopus laevis ribosomal DNACell, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevisJournal of Molecular Biology, 1976
- Morphology of transcriptional units of rDNAExperimental Cell Research, 1976
- A comparison of the structural organization of amplified ribosomal DNA from Xenopus mulleri and Xenopus laevisJournal of Molecular Biology, 1975