Evaluation of Ethylenediaminetetraacetic Acid, Tetrasodium Salt Dihydrate (EDTA)-Tween 20 Treatment versus Protease Digestion of Formalin-fixed Tissue Sections for Detection of Bovine Respiratory Syncytial Virus Antigen in Infected Ovine Lung
- 1 July 1989
- journal article
- research article
- Published by SAGE Publications in Veterinary Pathology
- Vol. 26 (4), 322-325
- https://doi.org/10.1177/030098588902600406
Abstract
The efficacy of protease and ethylenediaminetetraacetic acid, tetrasodium salt dihydrate (EDTA)-Tween 20 in unmasking bovine respiratory syncytial virus (BRSV) antigens in formalin-fixed lung tissue was compared using avidin-biotin immunoperoxidase procedure. Tissues were taken from experimentally infected lambs. BRSV antigen stained in both techniques. Treatment with EDTA-Tween 20 resulted in more intense staining of BRSV infected cells, more uniform cytoplasmic staining, less non-specific background, and superior cellular detail in comparison to protease digestion.Keywords
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