3H-Spiroperidol Binding Sites in the Rabbit Superior Mesenteric Artery

Abstract

The distribution of ³H-spiroperidol binding sites within rabbit superior mesenteric artery was studied in normal as well as 6-hydroxydopamine (6-OHDA) sympathectomized animals using a histoautoradiographic technique. The labelled drug was located in the three layers of the artery (adventitia, media and intima), with the greater density in the media. In the adventitia the radiolabelled drug was located at the level of fibrous and connective cells. In the media ³H-spiroperidol was bound by smooth muscle cells and is found in the cellular membrane of the same cells. 6-OHDA administration causes an increase in the number of ³H-spiroperidol binding sites in the adventitia and as well as a 20–25% increase in the media. The possible existence of a direct dopaminergic innervation of the superior mesenteric artery is discussed.