Lymphocyte transformation induced by autologous cells. XII. Quantitative and qualitative differences between human autologous and allogeneic reactive T lymphocytes.
- 1 April 1982
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 128 (4), 1723-1727
- https://doi.org/10.4049/jimmunol.128.4.1723
Abstract
The T cell populations that respond to autologous or allogeneic non-T cells are distinct, at least to a considerable extent. This conclusion is based on the differences in a) the size of the two populations; on the average one in 260 (individual frequency range, 1:149 to 1:417) T cells respond to allogeneic non-T cells whereas one in 3100 (individual frequency range, 1:2200 to 1:5000) T cells respond to autologous non-T cells; b) the phenotype of T cells activated in the MLR; autoactivated T cells were drawn predominantly from the OKT4-reactive T cell subpopulation, whereas alloactivated T cells activated in the allogeneic MLR were drawn from the OKT4, OKT5, and OKT8-reactive T cell subpopulations; and c) the specificity of activated and nonactivated T cells obtained on Percoll gradients after autologous or allogeneic MLR. Auto- or allodepleted T cells recovered from the 55 to 60% Percoll interface retained full alloreactivity or autoreactivity, respectively. However, auto- or alloactivated T cells recovered from the 40 to 50% Percoll interface showed secondary kinetics to allogeneic and autologous non-T cells. Whether the alloreactivity or autoreactivity found in autoactivated or alloactivated T cells, respectively, represents true cross-reacting T cells or T cells nonspecifically recruited during the MLR cannot at present be decided.This publication has 14 references indexed in Scilit:
- Surface phenotype of responder cells in the syngeneic mixed lymphocyte reaction in miceCellular Immunology, 1980
- Separation of functional subsets of human T cells by a monoclonal antibody.Proceedings of the National Academy of Sciences, 1979
- Purification of human T and B cells by a discontinuous density gradient of percollJournal of Immunological Methods, 1979
- Specificity and Suppressor Function of Human T Cells Responsive to Autologous Non-T CellsThe Journal of Immunology, 1979
- Restimulation in Secondary MLC by Autologous Non-T CellsScandinavian Journal of Immunology, 1979
- NATURE OF THE STIMULATORY CELL IN HUMAN ALLOGENEIC AND AUTOLOGOUS MLC REACTIONS - ROLE OF ISOLATED IGM-BEARING B-CELLS1979
- Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll)Analytical Biochemistry, 1978
- Lymphocyte transformation induced by autologous cells. V. generation of immunologic memory and specificity during the autologous mixed lymphocyte reactionThe Journal of Experimental Medicine, 1977
- Suppression of the Human Autologous Mixed Lymphocyte Reaction by Physiologic Concentrations of HydrocortisoneThe Journal of Immunology, 1977
- Stimulation of T Lymphocytes by Autologous non‐T Lymphoid Cells. Participation of HLA‐D?Tissue Antigens, 1977