Amino acid sequence surrounding the lipoic acid cofactor of bovine kidney 2‐oxoglutarate dehydrogenase complex
- 28 September 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 222 (1), 211-214
- https://doi.org/10.1016/0014-5793(87)80221-1
Abstract
The 2-oxoglutarate dehydrogenase complex was succinylated using 2-oxo[5-14C]glutarate in the presence of N-ethylmaleimide to label the lipoic acid cofactor of the transuccinylase (E2) component. Following peptic digestion, 14C-lipoate-containing peptides were purified and subjected to automated Edman degradation and amino acid analysis. The amino acid sequence surrounding the lipoyllysine residue is reported.Keywords
This publication has 12 references indexed in Scilit:
- Primary structure around the lipoate-attachment site on the E2 component of bovine heart pyruvate dehydrogenase complexBiochemical Journal, 1987
- The mammalian 2-oxoacid dehydrogenases: a complex familyTrends in Biochemical Sciences, 1986
- Ca2+ as a second messenger within mitochondriaTrends in Biochemical Sciences, 1986
- Nucleotide sequence of the sucA gene encoding the 2‐oxoglutarate dehydrogenase of Escherichia coli K12European Journal of Biochemistry, 1984
- Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferaseEuropean Journal of Biochemistry, 1984
- The Pyruvate Dehydrogenase Complex of Escherichia coli K12European Journal of Biochemistry, 1983
- Conformational mobility of polypeptide chains in the 2‐oxo acid dehydrogenase complexes from ox heart revealed by proton NMR spectroscopyFEBS Letters, 1981
- Purification of 2-oxo acid dehydrogenase multienzyme complexes from ox heart by a new methodBiochemical Journal, 1980
- Multienzyme complexesAccounts of Chemical Research, 1974
- α-Keto acid dehydrogenase complexesArchives of Biochemistry and Biophysics, 1972