Uptake and degradation of mast-cell granules by mouse peritoneal macrophages
- 15 July 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 182 (1), 189-193
- https://doi.org/10.1042/bj1820189
Abstract
35S-labelled mast-cell granules isolated from mouse mastocytomas were added to mouse macrophages in vitro. The granules were avidly phagocytosed, and subsequently the radioactivity was released to the medium as inorganic [35S]sulphate. After pulse-labelling, a total of about 80% of the cell-associated radioactivity was thus released in the course of 24 h, indicating an extensive breakdown of the sulphated polysaccharides, mainly heparin, present in the granules. The uptake of the mast-cell granules caused pronounced, but reversible, spreading of the macrophages.This publication has 13 references indexed in Scilit:
- Increased uptake and desulphaton of heparin by mouse macrophages in the presence of polycationsBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Distribution of sulfated mucopolysaccharides in invertebrates.Journal of Biological Chemistry, 1977
- Immunologic Release of Heparin from Purified Rat Peritoneal Mast CellsThe Journal of Immunology, 1977
- Chemistry of the hemostatic mechanism and its relationship to the action of heparin.1977
- Formation of anhydrosugars in the chemical depolymerization of heparinBiochemistry, 1976
- The biochemical basis for mucopolysaccharidoses and mucolipidoses.1974
- Degradation of heparin in mouse mastocytoma tissueBiochemical Journal, 1971
- THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTESThe Journal of Experimental Medicine, 1965
- A modified uronic acid carbazole reactionAnalytical Biochemistry, 1962
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951