The spectrophotometric determination of hydroxyproline: an analytical investigation

Abstract

Thirty-eight methods for the spectrophotometric determination of hydroxyproline have been tested. The sensitivity of twenty-seven of these under routine conditions and their applicability for determining of hydroxyproline in synthetic and natural mixtures have been examined. Most procedures were suitable for use in the presence of other amino-acids, but sugars, urea and ammonium chloride caused considerable analytical losses. No single method was both sensitive and reproducible for use with synthetic mixtures and biological materials. The best results with any method were obtained by the identical treatment of all samples and by use of internal standards.